Journal article
A protective vaccine delivery system for in vivo T cell stimulation using nanoengineered polymer hydrogel capsules
A Sexton, PG Whitney, SF Chong, AN Zelikin, APR Johnston, RD Rose, AG Brooks, F Caruso, SJ Kent
ACS Nano | Published : 2009
DOI: 10.1021/nn900715g
Abstract
Successful delivery of labile vaccine antigens, such as peptides and proteins, to stimulate CD4 and CD8 T cell immunity could improve vaccine strategies against chronic infections such as HIV and Hepatitis C. Layer-by-layer (LbL)-assembled nanoengineered hydrogel capsules represent a novel and promising technology for the protection and delivery of labile vaccine candidates to antigen-presenting cells (APCs). Here we report on the in vitro and in vivo immunostimulatory capabilities of LbL-assembled disulfide cross-linked poly(methacrylic acid) (PMASH) hydrogel capsules as a delivery strategy for protein and peptide vaccines using robust transgenic mice models and ovalbumin (OVA) as a model v..
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Funding Acknowledgements
We thank staff at the Microbiology and Immunology animal facility at The University of Melbourne. This work is supported by National Health and Medical Research Council grants (S.J.K), Australian Research Council grants under the Federation Fellowship (F.C.) and Discovery Project (F.C., A.P.R.J., A.N.Z.) schemes, and The University of Melbourne Strategic Research Infrastructure Fund (F.C., S.J.X).